Human pancreatic microcyst and duct-β-cell cluster (2025)

====  Antifade human pancreas polymerization established in Nat. Commun 2023  ====

Team-based human pancreas analysis in Taiwan (2022)

In the past five years, we has successfully transformed our 3-D imaging platform from mouse to human pancreas characterization.  We integrate the fields of surgery, pathology, and modern 3-D histology to investigate the human pancreas in health and disease.  Our ongoing work includes: (i) use of organ-wide PanIN and microadenoma analyses to elucidate exocrine-endocrine relationship in early pancreas remodeling and (ii) use of modern 3-D histology (integration of vibratome and microtome histology) to establish an image database of early lesions in the human pancreas.

Low-grade duct lesion (PanIN) in human donor pancreas (2022)

Associated human islet and duct remodeling (2022)

Article:  Local islet remodelling associated with duct lesion–islet complex in adult human pancreas (2021 Diabetologia)

Review:  Pancreas optical clearing and 3-D microscopy in health and diabetes (2021 Frontiers in Endocrinology)

Human pancreatic fatty infiltration. Images were derived from tile scanning of optically cleared pancreatic specimens. (A-B) Normal lobule of human pancreas. Adipocytes are clearly seen around the blood vessel and inside the lobule (magnified, arrows). Green, nuclear staining; red, CD31. (C-D) Acinar atrophy of diseased lobule. This view was acquired 2-cm distal to the pancreatic ductal adenocarcinoma. Overlay of transmitted light and fluorescence signals identifies the fatty infiltration.

​Article:  Autonomic innervation of human pancreas (2019 AJP-GI)

Article:  Lymphatic vessel remodeling and invasion in pancreatic cancer progression (2019 EBioMedicine - The Lancet)
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Association of PanIN and lymphatic network in surgical biopsy acquired from PDAC treatment.  The H&E image in inset i shows high-grade PanIN in the tumor bulk (male/age, 67 years/T3N0).  2-D fluorescence image (inset ii) and 3-D projection of signals (inset iii) identify the peri-PanIN lymphatic network.  Blue: islets (glucagon staining).  Green: nuclei.  Yellow arrows indicate the areas with aggregation of nuclear signals (inflammation), which appear to be associated with the lymphatic network (magenta, D2-40).

3-D multiplex histology of human pancreas (2020 DDW abstract)

Integration of transmitted light, fluorescence, and H&E signals to identify neuro-insular network in PanIN microenvironment.  A PanIN lesion was detected in the vibratome section of human pancreas (circle, A) and confirmed with the microtome-based H&E histology (B, gold standard).  The peri-lesional adipocyte association (transmitted light signals), islet aggregation (yellow, glucagon staining), and neuro-insular association (white in C & D, PGP9.5) are revealed via 3-D imaging with tissue clearing.

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​Islet aggregation around early duct lesions in human donor pancreas (American Diabetes Association 2019 Scientific Sessions) 

Human pancreatic neuro-insular network in health and fatty infiltration (2018 Diabetologia)

Panoramic view of pancreatic glial network and intra-pancreatic ganglia.  Cyan: S100B. Red: CD31. Yellow: nuclei.  This video shows a peri-lobular ganglion at the beginning (00:00-00:06, left side of the image stack), then the view moves to an islet and its nearby intra-parenchymal ganglion (00:09-00:12).  Dimension: 3,600×2,100×300 μm.

​Human pancreatic neuro-insular network (American Diabetes Association 2017 Scientific Sessions)