Panoramic-to-3D/super-resolution imaging in antifade polymer (slide sharing with excellent quality)
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The antifade Solid-SuperR® kidney slide (based on A-ha technology) features a fluorescently labeled mouse renal specimen embedded in the high-refractive-index polymer (n = 1.53; thickness: 350 ± 50 µm).
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The high-n polymer Solid-SuperR® facilitates photon penetration in 3D microscopy while the solvent-free condition minimizes photobleaching caused by oxygen-fluorophore contacts and reactions. Because of the unique stability, the Solid-SuperR® kidney slide is recommended for demonstration of 3D high/super-resolution imaging in the microscope center or imaging core. It can also serve as a teaching tool or positive control for routine confocal or fluorescence microscopy. Email us at 3d.histology.tw@gmail.com if you are interested in receiving the Solid-SuperR® kidney polymer slide.
Labeling specifics:
- DAPI labeling of nuclei (ex. peak: 350 nm; em. peak: 465 nm).
- WGA-AF555: cardiac perfusion of wheat germ agglutinin (WGA) -Alexa Fluor® 555 conjugates (ex. peak: 555 nm; em. peak: 565 nm); target: renal vessels (e.g., glomerulus capillaries) and basement membrane (WGA-AF555 across the capillaries).
- TUJ1-AF647: nerve fibers and bundles labeled with anti-tubulin beta 3 (TUBB3, clone TUJ1) -Alexa Fluor® 647 conjugates (ex. peak: 650 nm; em. peak: 665 nm).
- DAPI labeling of nuclei (ex. peak: 350 nm; em. peak: 465 nm).
- WGA-AF555: cardiac perfusion of wheat germ agglutinin (WGA) -Alexa Fluor® 555 conjugates (ex. peak: 555 nm; em. peak: 565 nm); target: renal vessels (e.g., glomerulus capillaries) and basement membrane (WGA-AF555 across the capillaries).
- TUJ1-AF647: nerve fibers and bundles labeled with anti-tubulin beta 3 (TUBB3, clone TUJ1) -Alexa Fluor® 647 conjugates (ex. peak: 650 nm; em. peak: 665 nm).
Panoramic-to-3D/super-resolution imaging of mouse kidney. Capillaries (red) and nuclei (white) of a glomerulus are closely associated. The two tissue/cellular components are excellent targets to demonstrate the resolving power of a 3D imaging approach (due to their close proximity). Hundreds of glomeruli are available in each Solid-SuperR® slide for magnification.
The transparent Solid-SuperR® can also serve as a standard slide to demonstrate 3D neurohistology (TUBB3/TUJ1 labeled, green). Please see YouTube videos for in-depth 3D/super-resolution imaging of renal glomerulus.
The transparent Solid-SuperR® can also serve as a standard slide to demonstrate 3D neurohistology (TUBB3/TUJ1 labeled, green). Please see YouTube videos for in-depth 3D/super-resolution imaging of renal glomerulus.
Reliable slide for 3D super-resolution imaging / presentation
Solid-SuperR® integrates with ZEISS Lattice SIM
Zeiss Lattice SIM 5 imaging of Solid-SuperR® mouse intestine